RESUMO
Samples from 45 chickens, two turkeys, one peacock and one quail with symptoms of fowlpox were collected in Mozambique between November 2016 and January 2018. Phylogenetic analysis revealed that the samples contained avipoxviruses belonging to both clade A1 and clade A2. In addition, all of the Clade A1 viruses were positive by PCR for the integration of reticuloendotheliosis virus, while the clade A2 avipoxvirus samples were negative. This study confirms the circulation of clade A1 avipoxviruses in Mozambique in addition to identifying clade A2 for the first time in the country.
Assuntos
Avipoxvirus/genética , Avipoxvirus/isolamento & purificação , Doenças das Aves/virologia , Infecções por Poxviridae/veterinária , Animais , Avipoxvirus/classificação , Galinhas , Varíola Aviária/virologia , Galliformes/virologia , Moçambique , Filogenia , Infecções por Poxviridae/virologia , Codorniz/virologia , Perus/virologiaRESUMO
Analysis of scab samples collected from poultry during outbreaks of fowlpox in Mozambique in 2016 revealed the presence of clade E avipoxviruses. Infected poultry were from flocks that had been vaccinated against fowlpox virus. These findings require urgent reevaluation of the vaccine formula and control strategies in this country.
Assuntos
Avipoxvirus/classificação , Vírus da Varíola das Aves Domésticas/classificação , Varíola Aviária/epidemiologia , Doenças das Aves Domésticas/epidemiologia , Infecções por Poxviridae/veterinária , Vacinação/veterinária , Animais , Avipoxvirus/genética , Avipoxvirus/isolamento & purificação , DNA Polimerase Dirigida por DNA/genética , DNA Polimerase Dirigida por DNA/metabolismo , Varíola Aviária/imunologia , Varíola Aviária/transmissão , Varíola Aviária/virologia , Vírus da Varíola das Aves Domésticas/genética , Vírus da Varíola das Aves Domésticas/isolamento & purificação , Expressão Gênica , Genótipo , Moçambique/epidemiologia , Filogenia , Aves Domésticas/virologia , Doenças das Aves Domésticas/imunologia , Doenças das Aves Domésticas/transmissão , Doenças das Aves Domésticas/virologia , Infecções por Poxviridae/epidemiologia , Infecções por Poxviridae/transmissão , Infecções por Poxviridae/virologia , Falha de Tratamento , Proteínas Virais/genética , Proteínas Virais/metabolismo , Vacinas Virais/administração & dosagemRESUMO
The complete sequence of the fusion (F) protein gene from 11 Newcastle disease viruses (NDVs) isolated from commercial poultry in Mozambique between 2011 and 2016 has been generated. The F gene cleavage site motif for all 11 isolates was (112)RRRKRF(117) indicating that the viruses are virulent. A phylogenetic analysis using the full F gene sequence revealed that the viruses clustered within genotype VIIh and showed a higher similarity to NDVs from South Africa, China and Southeast Asia than to viruses previously described in Mozambique in 1994, 1995 and 2005. The identification of these new NDVs has important implications for Newcastle disease management and control in Mozambique.
